Royal College of Surgeons in Ireland Coláiste Ríoga na Máinleá in Éirinn

Molecular & Cellular Imaging

The Cellular and Molecular Imaging core facility at RCSI mainly focuses on live cell imaging applications.

The core employs confocal GFP imaging, fluorescence resonance energy transfer (FRET), fluorescence recovery after photo bleaching (FRAP), FLIP and photo-activation/photo-conversion techniques also in combination with patch clamp. It support users from different fields in biomedical research.

In cases of sophisticated applications the imaging core get involved in research projects, and further develop microscopic imaging applications to enhance the quality of the output of the state of the art image acquisition setups based here.

Due to generous funding of the HEA, SFI, HRB, Cancer Research Ireland, and EU the core facility hosts: 

  • Two epi-fluorescence microscopes with CCD camera 
  • Three live cell epi-fluorescence imaging setups all suitable for fluorescence time lapse imaging under physiological conditions. One is additionally equipped with a microinjection system.
  • Four confocal microscopes equipped for live cell imaging applications
  • One high content screening platform also equipped for live cell applications.
  • IT Infrastructure for image storage, image processing and analysis

The core facility provides training in basic epi-fluorescence and confocal microscopy applications, image processing, and advanced live cell imaging.

For further information and requests please contact: Heiko Düssmann.